酶联免疫斑点技术(ELISPOT)::德国AID 专业的ELISPOT技术服务商

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ELISPOT全名为酶联免疫斑点检测，英文：Enzyme-linked Immunospot Assay。它结合了细胞培养技术与酶联免疫吸附技术（即ELISA技术），是从单细胞水平检测分泌抗体细胞(ASC) 或分泌细胞因子(CK) 细胞的一项细胞免疫学检测技术。ELISPOT技术原理就是用抗体捕获培养中的细胞分泌的细胞因子，并以酶联斑点显色的方式将其表现出来。由于ELISPOT方法具有较高的特异性和敏感性，易操作，成本相对流式细胞分析术也较低，已被广泛用于分泌CK细胞检测或ASC测定中，对探索自身免疫系统**发病机制具有重要意义。 

 

德国AID （Autoimmun Diagnostika GmbH），是全球**的Elispot技术服务提供商，为您的Elispot科研提供高效的分析工具--Elispot Reader (酶联免疫斑点图像分析仪)，Elispot Software（酶联斑点计数分析软件）以及Elispot kits（酶联斑点分析试剂盒）；并能够为您提供更加专业的Elispot技术服务，如Elispot读板检测服务，Elispot分析方案设计，Elispot技术交流，Elispot研讨讲座等，为您的Elispot科研提供更加科学系统的解决方案。

 


 


         		 
 
AID experience counts!
 
   
 

 

Be prepared for theEliSpotfutureandanalyse your enzymatic assays and yourmulti-colorFluoroSpotassaysin the same innovative AIDReader.

Scanningandcountingandanalysing withonlyonesoftware in justtwominutesin the  enzymaticmode.Fluorospot-count injust6-7minutes

  • Custom-made,softwaredrivenfiltercube with 8 positions(7& 1 type)
  • Enzymatic EliSpotassayswithanysubstrat and any type of 96-well plate
  • FluoroSpot assays with amaximumof21different fluorescent dyes
  • Only one mouse-clickfromenzymatictofluorescent scanning and vice versa
  • PreparedforCy-3/FITCtwo-coloranalysis, other filter sets on demand
  • Sophisticatednewimagingtechnologyto detect single- and double-stained spotswith100%accuracy
  • Achieves the samesensitivityaswithenzymatic based assays

FAQ on iSpot and FluoroSpot

 
   
 

WhyFluorescentEliSpot?

TheEliSpot assay isknowntobeone of the most sensitive cellularimmunoassaysavailable,allowingthe detection of cytokineproducing T-cells evenat thesingle celllevel. So far, onelimitation in EliSpot technologyhasbeen the lackto detectT-cells which simultaneouslyproducemultiplecytokines.

Toovercomethisproblemresearches have begun to develop EliSpotassays basedonfluorescentlabeling. Detection of multiplecytokinessecretingexpressinglymphocytes such as doubleIFN-γ/IL-2 producingT-cellswith thisnewEliSpot(FluoroSpot)-technology could provideadditionalandnewinformations regarding the clinical outcomeinpatientswithvarious viral infections and other questions.

  • advancedTH1/TH2analysis(e.g.Interferonvs.IL-2 and IL-10)
  • moredetailedanalysisofTcellsubpopulations
  • improved characterisation of shifted immuneresponseinvariousinfectious diseases
    γ-IFN assay done with traditional enzymatic AID EliSpot Reader
    γ-IFN assay done with fluorescent AID EliSpot Reader

IFN-γassaydonewithtraditional enzymatic (left)andwithfluorescent-basedEliSpot/FluoroSpot (right) andanalysedwiththe AID EliSpotsoftware. Sensitivity of bothassays isthesame, as the totalnumber of spots in both wellsisnearlyidentical.

 

Why AID iSpot?

AID has developed an unique EliSpot devicecapabletoanalyseFluorospot as well astraditionalenzymatic-basedEliSpotassays.

  • SincecommerciallyavailablevalidatedFluoroSpot assays are on the marketsince summer2008butare still inits development, it is reasonableto useahybrid-technolgy, whichallows tointerpreteenzymatic-andfluorescent-based assays with onlyonedevice.
  • ThenewAIDiSpotReaderisstill as fast as theoriginal AID Readers andcompletelyinterpretesa96-wellfluorescent plate within 6 –7minutes.
  • TheAIDiSpotReadercan switch betweenbothapproaches without hardware changesandwithout loss ofsensitivityin the assay analysis.
  • Thesophisticatednewoverlay-technologyallows for detailedinterpretationofdouble-expressing T-cells inevery well. Theadaptedfluorescentinterpretation software especiallyfacilitatesworkingwith thesesubtypes of cells.
  • Multiplecytokinedetectioninone well with up to 7 narrow band fluorescentfilter duetothecustom-made 7&1 AID filter cube.
  • The AID iSpot readercomprisesthesameoutstandingfunctionality,suchaslayout-generator,rule-compiler,various exportpossibilitesetc,asthe well known AID EliSpot ReaderSystems.
image A, Cy-3
image B, FITC
A.
B.
overlapped image C
comparison matrix image D
C.
D.

Fluorescent-based two-color IFN-γ/Il-2-assay interpretedwiththeAIDEliSpot software: The AID reader captures theCy-3/IL-2signal(A)before capturing the FITC/IFN-γ signal(B).Thesoftwareoverlays both images (C) and createsanartificialimagehighlighting the double-stained spots (D).

With the forward-looking AID technology Elispotresearcherswillsoonbe able to do real multi-color FluoroSpot assaysaswell.TheAIDiSpot is equipped with a 7&1 filtercubewhichtheoreticallyallows analysis of a maximum of 21differentdyes inonewell. Work is already in progress withthreedifferentdyes perwell.

   


On the following pages you will find information ontheAIDElispotReader Systems and the Elispot assays developedandproducedbyAID.

 

 

 

 

 

 
     
AutoimmunDiagnostikaGmbH
 Ebinger Strasse 4
D-72479 Strassberg
Germany
      		 

 

 

 
 
For further informations onotherAIDproducts,suchasmoleculardiagnostic kits,immunoblots,Hep-2screeningkits,automatedColony counters, Zonereaders,Biochipreaders andfully automated microscopes pleasevisitourotherwebsites:		 
 

www.aid-diagnostika.com
www.aid-china.com
www.aid-china.com
www.colony-counter.com